Cendawan susu harimau boleh mencairkan darah dan menghalang pembekuan darah.
Apakah ini bermakna cendawan ini sesuai untuk mereka yang mempunyai masalah jantung??
Berikut adalah sebahagian daripada kertas kajian yang dihasilkan oleh Prof Dr Vikineswary Sabaratnam.
Lignosus rhinocerotis
The tiger milk mushroom (L.rhinocerotis) is an indigenous mushroom of Malaysia and it is used by the indigenous communities of Malaysia to cure a number of diseases as well as for overall wellness.
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Recently, this mushroom has attracted much attention and a number of publications are available [11,12,13]. In this study, it is reported for the first time, the fibrinolytic and anticoagulant activities of crude extracts of L.rhinocerotis.
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The fibrinolytic activity of the sclerotium of L.rhinocerotis was shown in the qualitative fibrin plate assay as well as in the quantitative method of Folin spectroscopy method. The specific activity of the crude extract was 56.91 U/mg.
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The ATP system was used to concentrate and recover the fibrinolytic enzymes from the freeze-dried sclerotium of L.rhinocerotis. After acetone extraction of the top phase of the ATPS to precipitate the protein, the specific activity of the partially purified extract increased to 155.08 U/mg protein with a purification fold of 2.7. The recovery of the enzyme in the top phase is preferred as the PEG (polymer) can significantly enhance the refolding of proteins to stabilise the activity [14]. The molecular weight of the partially purified enzyme was approximately 45 kDa.
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The anticoagulant activity of extracts of the sclerotium of L.rhinocerotis was evaluated by the in-vitro anti-platelet activity assay [15]. The ATPS and acetone extraction was used to precipitate the protein of interest.
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The SDS–PAGE analysis showed that the protein with the anticoagulant activity had a molecular weight of approximately 45-50kDa. This result is similar to the protein from A. auricula-judae which had both fibrinolytic as well as anticoagulant activity (unpublished data).
.
Recently, this mushroom has attracted much attention and a number of publications are available [11,12,13]. In this study, it is reported for the first time, the fibrinolytic and anticoagulant activities of crude extracts of L.rhinocerotis.
.
The fibrinolytic activity of the sclerotium of L.rhinocerotis was shown in the qualitative fibrin plate assay as well as in the quantitative method of Folin spectroscopy method. The specific activity of the crude extract was 56.91 U/mg.
.
The ATP system was used to concentrate and recover the fibrinolytic enzymes from the freeze-dried sclerotium of L.rhinocerotis. After acetone extraction of the top phase of the ATPS to precipitate the protein, the specific activity of the partially purified extract increased to 155.08 U/mg protein with a purification fold of 2.7. The recovery of the enzyme in the top phase is preferred as the PEG (polymer) can significantly enhance the refolding of proteins to stabilise the activity [14]. The molecular weight of the partially purified enzyme was approximately 45 kDa.
.
The anticoagulant activity of extracts of the sclerotium of L.rhinocerotis was evaluated by the in-vitro anti-platelet activity assay [15]. The ATPS and acetone extraction was used to precipitate the protein of interest.
.
The SDS–PAGE analysis showed that the protein with the anticoagulant activity had a molecular weight of approximately 45-50kDa. This result is similar to the protein from A. auricula-judae which had both fibrinolytic as well as anticoagulant activity (unpublished data).
(sumber: FIBRINOLYTIC AND ANTICOAGULANT ENZYMES OF SELECTED MEDICINAL AND CULINARY MUSHROOMS, V.SABARATNAM)
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